I concur with Biffo that you would be unlikely to confuse "No." Together with the word "No" when you concentrate on it. But I do at times experience a break up-next hesitation when looking through a sentence with "No.", particularly if there's a House among it and the subsequent number: https://andresdthwg.mybloglicious.com/56509982/helping-the-others-realize-the-advantages-of-no-scrubs-tlc